Cellular Microenvironment Dictates Androgen Production by Murine Fetal Leydig Cells in Primary Culture1
نویسندگان
چکیده
منابع مشابه
Effect of Androgen on Proliferation of Estrogen-responsive Transformed Mouse Leydig Cells in Serum-free Culture1
We examined the effects of steroid hormones on the proliferation of transformed mouse Leydig cells (B-l) in serum-free culture condition. Among hormones examined, androgen as well as estrogen enhanced the cell proliferation rate. Hormone binding studies revealed that B-l cells contained both androgen and estrogen receptors. In addition, androgenenhanced cell growth was inhibited by antiandrogen...
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Wilms' tumor 1 (Wt1) is a tumor suppressor gene encoding ∼24 zinc finger transcription factors. In the mammalian testis, Wt1 is expressed mostly by Sertoli cells (SCs) involved in testis development, spermatogenesis, and adult Leydig cell (ALC) steroidogenesis. Global knockout (KO) of Wt1 is lethal in mice due to defects in embryogenesis. Herein, we showed that Wt1 is involved in regulating fet...
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Disruption of normal fetal development can influence functioning of organs and cells in adulthood. Circumstantial evidence suggests that subtle reductions in fetal androgen production may be the cause of adult male reproductive disorders due to reduced testosterone production. The mechanisms through which these fetal events affect adult testosterone levels are largely unknown. A recent paper of...
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In contrast to the strong stimulation of testosterone production by hCG, L-isoproterenol had little effect on freshly isolated Leydig cells from 18-day-old mouse fetuses. However, the ability of fetal Leydig cells to respond to L-isoproterenol exposure increased during culture (0-24 h). The response of the cultured cells to L-isoproterenol was dose-dependent with an ED50 at 2 X 10(-7) M. Adrena...
متن کاملExposure to Perfluorooctane Sulfonate In Utero Reduces Testosterone Production in Rat Fetal Leydig Cells
BACKGROUND Perfluorooctane sulfonate (PFOS) is a synthetic material that has been widely used in industrial applications for decades. Exposure to PFOS has been associated with decreased adult testosterone level, and Leydig cell impairment during the time of adulthood. However, little is known about PFOS effects in utero on fetal Leydig cells (FLC). METHODS AND RESULTS The present study invest...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 2014
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod.114.118570